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TFF1 Polyclonal antibody

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13734-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Protein pS2, Polypeptide P1.A, PNR-2, pNR 2, HP1.A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman stomach tissue, human kidney tissue, mouse stomach tissue
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, human ovary tumor tissue, human stomach tissue, mouse stomach tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TFF1 Ready-To-Use IHC Kit. TFF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13734-1-AP targets TFF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 TFF1 fusion protein Ag4541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trefoil factor 1
别名 Protein pS2, Polypeptide P1.A, PNR-2, pNR 2, HP1.A
计算分子量 84 aa, 9 kDa
观测分子量 9-12 kDa
GenBank蛋白编号BC032811
基因名称 TFF1
Gene ID (NCBI) 7031
RRIDAB_2202853
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04155
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

实验方案

Product Specific Protocols
WB protocol for TFF1 antibody 13734-1-APDownload protocol
IHC protocol for TFF1 antibody 13734-1-APDownload protocol
IF protocol for TFF1 antibody 13734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Exp Med

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Authors - Yalong Wang
humanIHC

JCI Insight

Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing.

Authors - Wei Guo
humanIHC, WB

Bone

Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis.

Authors - Chiara Spadazzi
  • KO Validated
humanWB, IHC

Biomed Pharmacother

Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers.

Authors - Jie Yi
  • KD Validated
humanWB

Aesthet Surg J

Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization.

Authors - Bijun Kang
humanIHC

BMC Med Genomics

Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection.

Authors - Kim Hark Kyun HK
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