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TFF1 Recombinant antibody, PBS Only (Capture)

TFF1 Uni-rAbTM Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240424F5

Cat no : 83467-2-PBS

Print datasheet

Synonyms

Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein

验证数据展示

  • FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

    FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83467-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00469-1

    Cytometric bead array standard curve of MP00469-1

    Cytometric bead array standard curve of MP00469-1, TFF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83467-2-PBS. Detection antibody: 83467-1-PBS. Standard: Ag4541. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83467-2-RR

    Affinity and Kinetic Characterization of 83467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

Planning an IHC experiment? We recommend our IHCeasy TFF1 Ready-To-Use IHC Kit. TFF1 primary antibody included.

产品信息

83467-2-PBS targets TFF1 as part of a matched antibody pair:

MP00469-1: 83467-2-PBS capture and 83467-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TFF1 fusion protein Ag4541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name trefoil factor 1
Synonyms Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein
Calculated Molecular Weight 84 aa, 9 kDa
GenBank Accession NumberBC032811
Gene Symbol TFF1
Gene ID (NCBI) 7031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04155
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.