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TFF1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 31.25-2000 pg/mL

Cat No : MP00469-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00469-2, TFF1 Recombinant Matched Antibody Pair - PBS only. 83467-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4541. 83467-3-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

    Sandwich ELISA standard curve of MP00469-2

    Sandwich ELISA standard curve of MP00469-2, TFF1 Recombinant Matched Antibody Pair - PBS only. 83467-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4541. 83467-3-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Serum of sixteen individual healthy human donors was measured. The TFF1 concentration of detected samples was determined to be 4.6 ng/mL with a range of 1.1 - 14.6 ng/mL.

    Sample test of MP00469-2

    Serum of sixteen individual healthy human donors was measured. The TFF1 concentration of detected samples was determined to be 4.6 ng/mL with a range of 1.1 - 14.6 ng/mL.

  • Urine of eight individual healthy human donors was measured. The TFF1 concentration of detected samples was determined to be 73.7 ng/mL with a range of 12.4 - 196.2 ng/mL.

    Sample test of MP00469-2

    Urine of eight individual healthy human donors was measured. The TFF1 concentration of detected samples was determined to be 73.7 ng/mL with a range of 12.4 - 196.2 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00469-2 targets TFF1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 31.25-2000 pg/mL (Sandwich ELISA);

Capture antibody: TFF1 Recombinant antibody, PBS Only (Capture/Detector) 83467-1-PBS (240424E6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: TFF1 Recombinant antibody, PBS Only (Detector) 83467-3-PBS (240424H4). 100 μg. Concentration 1 mgl/ml.

Alternative TFF1 matched antibody pairs: MP00469-1, MP50027-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83467-1-PBS
Tested Reactivity human
Immunogen TFF1 fusion protein Ag4541
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name trefoil factor 1
Synonyms Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein
GenBank accession numberBC032811
Gene symbol TFF1
UniprotP04155
Gene ID (NCBI) 7031
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83467-3-PBS
Tested Reactivity human
Immunogen TFF1 fusion protein Ag4541
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name trefoil factor 1
Synonyms Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein
GenBank accession numberBC032811
Gene symbol TFF1
UniprotP04155
Gene ID (NCBI) 7031
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C