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验证数据展示

  • WB analysis of human urine using 83717-4-RR (same clone as 83717-4-PBS)

    WB analysis of human urine using 83717-4-RR (same clone as 83717-4-PBS)

    Human urine sample was subjected to SDS PAGE followed by western blot with 83717-4-RR (TFF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83717-4-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 83717-4-RR (same clone as 83717-4-PBS)

    FC experiment of PC-3 using 83717-4-RR (same clone as 83717-4-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug TFF2 Recombinant antibody (83717-4-RR, Clone:240661G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83717-4-PBS in a different storage buffer formulation.

  • IP experiment of mouse stomach using 83717-4-RR (same clone as 83717-4-PBS)

    IP experiment of mouse stomach using 83717-4-RR (same clone as 83717-4-PBS)

    IP result of anti-TFF2 (IP:83717-4-RR, 4ug; Detection:83717-4-RR 1:1000) with mouse stomach tissue lysate 1360 ug. This data was developed using the same antibody clone with 83717-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83717-4-RR

    Affinity and Kinetic Characterization of 83717-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83717-4-RR against Human TFF2 were performed. The affinity constant is below 1 pM.

TFF2 Recombinant antibody, PBS Only

TFF2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 83717-4-PBS

产品说明书

CloneNo. 240661G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, ELISA

别名

SML1, 240661G12, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83717-4-PBS targets TFF2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 TFF2 fusion protein Ag4537 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trefoil factor 2
别名 SML1, 240661G12, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin
计算分子量 129 aa, 14 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC032820
基因名称 TFF2
Gene ID (NCBI) 7032
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ03403
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).