验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23277-1-AP targets Trefoil factor 3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Trefoil factor 3 fusion protein Ag9543 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trefoil factor 3 (intestinal) |
| 别名 | HITF, hP1.B, Intestinal trefoil factor, ITF, Polypeptide P1.B, TFF3, TFI, Trefoil factor 3, trefoil factor 3 (intestinal) |
| 计算分子量 | 80 aa, 9 kDa |
| GenBank蛋白编号 | BC017859 |
| 基因名称 | TFF3 |
| Gene ID (NCBI) | 7033 |
| RRID | AB_2879245 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q07654 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Trefoil factor 3 (TFF3) belongs to the TFF-domain peptide family which consists of three small secreted proteins (TFF1, TFF2, TFF3) that are expressed by mucous-secreting epithelia. TFF3 is a major constituent in the goblet cells in small and large intestines, especially a typical secretary peptide of the normal human antral and pyloric gastric mucosa. TFF3 is essential in regulating cell migration and maintaining normal GI mucosal integrity. TFF3 has been reported to be overexpressed at the gene and the protein level in human neoplasms such as prostate, breast, and colon cancer. TFF3 is involved in tumor cell scattering, angiogenesis, and invasion.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Trefoil factor 3 antibody 23277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Histotechnol Trefoil factor 3 knock-down prevents autophagy-related gene 12 elevation in colon adenocarcinoma.
| ||
Biomark Res Spatial resolved transcriptomics reveals distinct cross-talk between cancer cells and tumor-associated macrophages in intrahepatic cholangiocarcinoma |
