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TFG Monoclonal antibody, PBS Only (Capture)

TFG Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66916-1-PBS

Print datasheet

Synonyms

TRKT3, TRK-fused gene protein, TRK fused gene protein, TRK fused gene, TF6

验证数据展示

  • WB analysis using 66916-1-Ig (same clone as 66916-1-PBS)

    WB analysis using 66916-1-Ig (same clone as 66916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50894-1

    Cytometric bead array standard curve of MP50894-1

    Cytometric bead array standard curve of MP50894-1, TFG Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66916-1-PBS. Detection antibody: 66916-2-PBS. Standard:Ag27697. Range: 0.391-200 ng/mL.

产品信息

66916-1-PBS targets TFG as part of a matched antibody pair:

MP50894-1: 66916-1-PBS capture and 66916-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen TFG fusion protein Ag27697 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name TRK-fused gene
Synonyms TRKT3, TRK-fused gene protein, TRK fused gene protein, TRK fused gene, TF6
Calculated Molecular Weight 400 aa, 43 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC023599
Gene Symbol TFG
Gene ID (NCBI) 10342
RRIDAB_2882243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92734
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein TFG (TRK-fused gene protein) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. Defects in TFG are a cause of thyroid papillary carcinoma (TPC), a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, and atrophy followed by distal sensory involvement. Recent genetic investigation indicates that formation of TFG-containing cytoplasmic inclusions and concomitant mislocalization of TAR DNA-binding protein 43 kDa (TDP-43) underlie motor neuron degeneration in HMSN-P. Pathological overlap of proteinopathies involving TFG and TDP-43 highlights a new pathway leading to motor neuron degeneration.