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TFIP11 Monoclonal antibody, PBS Only

TFIP11 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A1C8

Cat No : 66114-1-PBS

Print datasheet

Synonyms

bK445C9.6, STIP, STIP 1, TFIP11, TIP39

验证数据展示

  • WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

    IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66114-1-Ig(TFIP11 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

    IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

产品信息

66114-1-PBS targets TFIP11 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity human
Immunogen TFIP11 fusion protein Ag5821 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tuftelin interacting protein 11
Synonyms bK445C9.6, STIP, STIP 1, TFIP11, TIP39
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBB9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.