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验证数据展示

  • WB analysis using 30689-1-AP

    WB analysis using 30689-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30689-1-AP (TFPI2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

TFPI2 Polyclonal antibody

TFPI2 Polyclonal Antibody for WB, ELISA
Cat No. 30689-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TFPI 2, REF1, PP5, Placental protein 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30689-1-AP targets TFPI2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TFPI2 fusion protein Ag33467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tissue factor pathway inhibitor 2
Synonyms TFPI 2, REF1, PP5, Placental protein 5
Calculated Molecular Weight27 kDa
Observed Molecular Weight27-36 kDa
GenBank Accession NumberNM_001271003.1
Gene Symbol TFPI2
Gene ID (NCBI) 7980
RRIDAB_3669750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48307
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFPI2 (tissue factor pathway inhibitor 2), also known as PP5. It is expected to be located in extracellular space, and the protein is enriched in the placenta. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotrypsin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. The molecular weight of TFPI2 is 27 kDa, and it can recognize the band of 36 kDa (PMID: 21421890).

实验方案

Product Specific Protocols
WB protocol for TFPI2 antibody 30689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols