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验证数据展示

  • WB analysis using 83167-5-RR (same clone as 83167-5-PBS)

    WB analysis using 83167-5-RR (same clone as 83167-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR (same clone as 83167-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR (same clone as 83167-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGFB2 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR (same clone as 83167-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR (same clone as 83167-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGFB2 antibody included
  • IF Staining of HeLa using 83167-5-RR (same clone as 83167-5-PBS)

    IF Staining of HeLa using 83167-5-RR (same clone as 83167-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGFB2-Specific antibody (83167-5-RR, Clone: 230461F5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83167-5-RR (same clone as 83167-5-PBS)

    FC experiment of A549 using 83167-5-RR (same clone as 83167-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Tgfb2-Specific Recombinant Antibody (83167-5-RR, Clone:230461F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

TGFB2/TGF-beta 2 Recombinant antibody, PBS Only

TGFB2/TGF-beta 2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83167-5-PBS

产品说明书

CloneNo. 230461F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

TGFB2-Specific, TGFB2, TGF-beta 2, TGF beta 2, TGF B2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TGFB2 Ready-To-Use IHC Kit. TGFB2 primary antibody included.

产品信息

83167-5-PBS targets TGFB2/TGF-beta 2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor, beta 2
别名 TGFB2-Specific, TGFB2, TGF-beta 2, TGF beta 2, TGF B2
计算分子量 48 kDa
观测分子量48 kDa
GenBank蛋白编号NM_003238
基因名称 TGFB2
Gene ID (NCBI) 7042
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61812
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.