验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, Daudi cells, HL-60 cells, Jurkat cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
产品信息
11522-1-AP targets TGIF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | TGIF2 fusion protein Ag2085 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TGFB-induced factor homeobox 2 |
| 别名 | TGF-beta-induced transcription factor 2, TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2 |
| 计算分子量 | 237 aa, 26 kDa |
| 观测分子量 | 28 kDa and 30 kDa |
| GenBank蛋白编号 | BC012816 |
| 基因名称 | TGIF2 |
| Gene ID (NCBI) | 60436 |
| RRID | AB_2202731 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZN2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transforming growth factor-beta-induced factor 2(TGIF2), is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein. This antibody detected two forms of bands of about 28 and 30 kDa, and the upper band appears to result from increased phosphorylation (PMID: 11427533).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TGIF2 antibody 11522-1-AP | Download protocol |
| IHC protocol for TGIF2 antibody 11522-1-AP | Download protocol |
| IF protocol for TGIF2 antibody 11522-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells. | ||
Biochem J The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. | ||
Exp Ther Med Molecular mechanisms in vascular injury induced by hypertension: Expression and role of microRNA-34a. | ||
Brain Behav Downregulation of TGIF2 is possibly correlated with neuronal apoptosis and autism-like symptoms in mice. | ||
Front Immunol TGIF2 is a potential biomarker for diagnosis and prognosis of glioma
|
