验证数据展示

WB analysis using 12912-3-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12912-3-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human cervix using 12912-3-AP
Immunohistochemical analysis of paraffin-embedded human cervix using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens).
WB analysis using 12912-3-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 12912-3-AP
IP result of anti-TGM1 (IP:12912-3-AP, 4ug; Detection:12912-3-AP 1:1000) with mouse kidney tissue lysate 4000ug.
IHC staining of human cervical cancer using 12912-3-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12912-3-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 12912-3-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12912-3-AP (TGM1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 12912-3-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12912-3-AP (TGM1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brown disease using 12912-3-AP
Immunohistochemical analysis of paraffin-embedded human brown disease slide using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TGM1 Polyclonal antibody

TGM1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12912-3-AP

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Publications(34)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA and More (1)

Synonyms

LI1, LI, KTG, ICR2, Epidermal TGase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (34)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	human saliva, mouse kidney tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human cervical cancer tissue, human bowen disease, human brown disease, human cervix tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 16 publications below
IHC	See 6 publications below
IF	See 11 publications below
ELISA	See 1 publications below

产品信息

12912-3-AP targets TGM1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, ELISA
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Immunogen	TGM1 fusion protein Ag3905 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
Synonyms	LI1, LI, KTG, ICR2, Epidermal TGase
Calculated Molecular Weight	817 aa, 90 kDa
Observed Molecular Weight	90 kDa
GenBank Accession Number	BC034699
Gene Symbol	TGM1
Gene ID (NCBI)	7051
RRID	AB_2271748
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22735
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Transglutaminase 1 is an epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelopes in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.

实验方案

Product Specific Protocols
WB protocol for TGM1 antibody 12912-3-AP	Download protocol
IHC protocol for TGM1 antibody 12912-3-AP	Download protocol
IP protocol for TGM1 antibody 12912-3-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
human	WB	J Control Release Superior COL7A1 and TGM1 gene expression in difficult-to-transfect skin cell mediated by highly branched poly(β-amino esters) through stepwise fractionation Authors - Chaolan Pan View Article
human	WB	J Invest Dermatol Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells. Authors - Mélissa Simard View Article
human	WB	J Invest Dermatol Cathelicidin LL-37 Activates Human Keratinocyte Autophagy through the P2X₇, Mechanistic Target of Rapamycin, and MAPK Pathways Authors - Risa Ikutama View Article
human	IF	Int J Mol Sci Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model. Authors - Andréa Tremblay View Article
mouse	WB,ELISA	Cells Increased Osteoclastogenesis in Absence of TG2 Is Reversed by Transglutaminase Inhibition-Evidence for the Role for TG1 in Osteoclast Formation Authors - Sahar Ebrahimi Samani View Article

验证数据展示

WB analysis using 12912-3-AP

WB analysis of mouse kidney using 12912-3-AP

IHC staining of human cervix using 12912-3-AP

WB analysis using 12912-3-AP

IP experiment of mouse kidney using 12912-3-AP

IHC staining of human cervical cancer using 12912-3-AP

IHC staining of human cervical cancer using 12912-3-AP

IHC staining of human bowen disease using 12912-3-AP

IHC staining of human bowen disease using 12912-3-AP

IHC staining of human brown disease using 12912-3-AP

TGM1 Polyclonal antibody

TGM1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Superior COL7A1 and TGM1 gene expression in difficult-to-transfect skin cell mediated by highly branched poly(β-amino esters) through stepwise fractionation

Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells.

Cathelicidin LL-37 Activates Human Keratinocyte Autophagy through the P2X₇, Mechanistic Target of Rapamycin, and MAPK Pathways

Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model.

Increased Osteoclastogenesis in Absence of TG2 Is Reversed by Transglutaminase Inhibition-Evidence for the Role for TG1 in Osteoclast Formation