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验证数据展示

  • WB analysis of human saliva using 67004-1-Ig

    WB analysis of human saliva using 67004-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 67004-1-Ig (TGM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67004-1-Ig

    IHC staining of human tonsillitis using 67004-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67004-1-Ig (TGM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TGM1 Monoclonal antibody

TGM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67004-1-Ig

产品说明书

CloneNo. 3D9C11
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human and More (1)

应用

WB, IHC, ELISA

别名

Epidermal TGase, ICR2, KTG, LI, LI1, TG(K), TGASE, TGase 1, TGase K, TGK, TGM1, Transglutaminase 1, Transglutaminase K

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman saliva tissue
Positive IHC detected inhuman tonsillitis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67004-1-Ig targets TGM1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human
文献引用反应性human
免疫原 TGM1 fusion protein Ag3905 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
别名 Epidermal TGase, ICR2, KTG, LI, LI1, TG(K), TGASE, TGase 1, TGase K, TGK, TGM1, Transglutaminase 1, Transglutaminase K
计算分子量 817 aa, 90 kDa
观测分子量 90 kDa
GenBank蛋白编号BC034699
基因名称 TGM1
Gene ID (NCBI) 7051
RRIDAB_2882321
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP22735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transglutaminase 1 is a epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelope in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.

实验方案

Product Specific Protocols
WB protocol for TGM1 antibody 67004-1-IgDownload protocol
IHC protocol for TGM1 antibody 67004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

Growth modulatory effects of fenretinide encompass keratinocyte terminal differentiation: A favorable outcome for oral squamous cell carcinoma chemoprevention

Authors - Daren Wang
View Article