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TGM2 Polyclonal antibody

TGM2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15100-1-AP

Print datasheet

Synonyms

G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H



经过测试的应用

Positive WB detected inHUVEC cells, HepG2 cells, BxPC-3 cells, HeLa cells, A549 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TGM2 Ready-To-Use IHC Kit. TGM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15100-1-AP targets TGM2 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, ChIP
Tested Reactivity human
Cited Reactivityhuman, mouse, canine
Immunogen TGM2 fusion protein Ag7439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Synonyms G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77-80 kDa
GenBank Accession NumberBC003551
Gene Symbol TGM2
Gene ID (NCBI) 7052
ENSEMBL Gene IDENSG00000198959
RRIDAB_2202885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21980
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM2 antibody 15100-1-APDownload protocol
IHC protocol for TGM2 antibody 15100-1-APDownload protocol
IP protocol for TGM2 antibody 15100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nature

Autoimmune amelogenesis imperfecta in patients with APS-1 and coeliac disease

Authors - Yael Gruper
canine,humanIF

Nat Genet

New genes helped acorn barnacles adapt to a sessile lifestyle

Authors - Zhaofang Han
humanWB,IF

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
humanWB,IF

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
humanWB

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
humanWB,IHC

Cell Oncol (Dordr)

Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer

Authors - Shan Zhang
  • KD Validated