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验证数据展示

  • WB analysis using 82552-4-RR

    WB analysis using 82552-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82552-4-RR (TGM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 82552-4-RR

    IHC staining of human stomach cancer using 82552-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82552-4-RR (TGM2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TGM2 antibody included
  • Affinity and Kinetic Characterization of 82552-4-RR

    Affinity and Kinetic Characterization of 82552-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82552-4-RR against Human TGM2 were performed. The affinity constant is below 1 pM.

TGM2 Recombinant antibody

TGM2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82552-4-RR

产品说明书

CloneNo. 242828D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

242828D3, Erythrocyte transglutaminase, TG(C), TG2, TGase 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, U2OS cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TGM2 Ready-To-Use IHC Kit. TGM2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82552-4-RR targets TGM2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 TGM2 fusion protein Ag7439 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
别名 242828D3, Erythrocyte transglutaminase, TG(C), TG2, TGase 2
计算分子量 77 kDa
观测分子量 77-80 kDa
GenBank蛋白编号BC003551
基因名称 TGM2
Gene ID (NCBI) 7052
ENSEMBL Gene IDENSG00000198959
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21980
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM2 antibody 82552-4-RRDownload protocol
IHC protocol for TGM2 antibody 82552-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols