验证数据展示
经过测试的应用
| Positive WB detected in | SMMC-7721 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21406-1-AP targets TGM3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TGM3 fusion protein Ag16131 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase) |
| 别名 | TG(E), TGase 3, TGase E, TGE, TGM3, Transglutaminase 3, Transglutaminase E |
| 计算分子量 | 693 aa, 77 kDa |
| 观测分子量 | 77 kDa; 30-35 kDa; 44-50 kDa |
| GenBank蛋白编号 | BC109076 |
| 基因名称 | TGM3 |
| Gene ID (NCBI) | 7053 |
| RRID | AB_3085655 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08188 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TGM3 (Transglutaminase 3) is a Protein Coding gene. Diseases associated with TGM3 include Uncombable Hair Syndrome 2 and Uncombable Hair Syndrome 1. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. TG3 is expressed as an inactive 77 kDa zymogen which must be activated by limited proteolytic processing into two fragments (44 kDa and 30 kDa) of which the larger, N-terminal fragment carries the catalytic activity (PMID: 35328331).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TGM3 antibody 21406-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |