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验证数据展示

  • WB analysis of mouse testis using 15847-1-AP

    WB analysis of mouse testis using 15847-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15847-1-AP

    WB analysis using 15847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15847-1-AP

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 15847-1-AP

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

TGM4 Polyclonal antibody

TGM4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15847-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, HeLa cells, PC-3 cells, rat testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15847-1-AP targets TGM4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TGM4 fusion protein Ag8608 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transglutaminase 4 (prostate)
别名 Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P
计算分子量 684 aa, 77 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC007003
基因名称 TGM4
Gene ID (NCBI) 7047
RRIDAB_2287328
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49221
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM4 antibody 15847-1-APDownload protocol
IHC protocol for TGM4 antibody 15847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols