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TGM4 Polyclonal antibody

TGM4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15847-1-AP

Print datasheet

Synonyms

Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P

验证数据展示

  • WB analysis of mouse testis using 15847-1-AP

    WB analysis of mouse testis using 15847-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15847-1-AP

    WB analysis using 15847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15847-1-AP

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 15847-1-AP

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, HeLa cells, PC-3 cells, rat testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15847-1-AP targets TGM4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TGM4 fusion protein Ag8608 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transglutaminase 4 (prostate)
Synonyms Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P
Calculated Molecular Weight 684 aa, 77 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC007003
Gene Symbol TGM4
Gene ID (NCBI) 7047
RRIDAB_2287328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM4 antibody 15847-1-APDownload protocol
IHC protocol for TGM4 antibody 15847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols