• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TGM5 Polyclonal antibody

TGM5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25071-1-AP

Print datasheet

Synonyms

TG(X), TGase 5, TGase X, TGM5, TGM6, TGMX, TGX, transglutaminase 5, Transglutaminase X

验证数据展示

  • WB analysis of mouse skin using 25071-1-AP

    WB analysis of mouse skin using 25071-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 25071-1-AP (TGM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skin using 25071-1-AP

    IP experiment of mouse skin using 25071-1-AP

    IP result of anti-TGM5 (IP:25071-1-AP, 4ug; Detection:25071-1-AP 1:500) with mouse skin tissue lysate 4000ug.

  • IHC staining of human skin using 25071-1-AP

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 25071-1-AP

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skin tissue, A431 cells
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25071-1-AP targets TGM5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TGM5 fusion protein Ag18492 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transglutaminase 5
Synonyms TG(X), TGase 5, TGase X, TGM5, TGM6, TGMX, TGX, transglutaminase 5, Transglutaminase X
Calculated Molecular Weight 720 aa, 81 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC122859
Gene Symbol TGM5
Gene ID (NCBI) 9333
RRIDAB_2879883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43548
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM5 antibody 25071-1-APDownload protocol
IHC protocol for TGM5 antibody 25071-1-APDownload protocol
IP protocol for TGM5 antibody 25071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols