• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TGM5 Polyclonal antibody

TGM5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25071-1-AP

Print datasheet

Synonyms

TG(X), TGase 5, TGase X, TGM5, TGM6, TGMX, TGX, transglutaminase 5, Transglutaminase X

验证数据展示

  • WB analysis of mouse skin using 25071-1-AP

    WB analysis of mouse skin using 25071-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 25071-1-AP (TGM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skin using 25071-1-AP

    IP experiment of mouse skin using 25071-1-AP

    IP result of anti-TGM5 (IP:25071-1-AP, 4ug; Detection:25071-1-AP 1:500) with mouse skin tissue lysate 4000ug.

  • IHC staining of human skin using 25071-1-AP

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 25071-1-AP

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skin tissue, A431 cells
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25071-1-AP is TGM5 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TGM5 fusion protein Ag18492 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transglutaminase 5
Synonyms TG(X), TGase 5, TGase X, TGM5, TGM6, TGMX, TGX, transglutaminase 5, Transglutaminase X
Calculated Molecular Weight 720 aa, 81 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC122859
Gene Symbol TGM5
Gene ID (NCBI) 9333
RRIDAB_2879883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43548
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM5 antibody 25071-1-APDownload protocol
IHC protocol for TGM5 antibody 25071-1-APDownload protocol
IP protocol for TGM5 antibody 25071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols