• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse skin using 25071-1-AP

    WB analysis of mouse skin using 25071-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 25071-1-AP (TGM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skin using 25071-1-AP

    IP experiment of mouse skin using 25071-1-AP

    IP result of anti-TGM5 (IP:25071-1-AP, 4ug; Detection:25071-1-AP 1:500) with mouse skin tissue lysate 4000ug.

  • IHC staining of human skin using 25071-1-AP

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 25071-1-AP

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 40x lens).

TGM5 Polyclonal antibody

TGM5 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25071-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

TG(X), TGase 5, TGase X, TGM5, TGM6, TGMX, TGX, transglutaminase 5, Transglutaminase X

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse skin tissue, A431 cells
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25071-1-AP targets TGM5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TGM5 fusion protein Ag18492 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transglutaminase 5
别名 TG(X), TGase 5, TGase X, TGM5, TGM6, TGMX, TGX, transglutaminase 5, Transglutaminase X
计算分子量 720 aa, 81 kDa
观测分子量 84 kDa
GenBank蛋白编号BC122859
基因名称 TGM5
Gene ID (NCBI) 9333
RRIDAB_2879883
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43548
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM5 antibody 25071-1-APDownload protocol
IHC protocol for TGM5 antibody 25071-1-APDownload protocol
IP protocol for TGM5 antibody 25071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols