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TH1L Monoclonal antibody, PBS Only

TH1L Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10G2

Cat no : 67380-1-PBS

Print datasheet

Synonyms

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

验证数据展示

  • WB analysis using 67380-1-Ig (same clone as 67380-1-PBS)

    WB analysis using 67380-1-Ig (same clone as 67380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

    IF Staining of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67380-1-Ig (TH1L antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

    FC experiment of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TH1L (67380-1-Ig, Clone:1A10G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NELFCD/TH1L Ready-To-Use IHC Kit. NELFCD/TH1L primary antibody included.

产品信息

67380-1-PBS targets TH1L in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen TH1L fusion protein Ag24894 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TH1-like (Drosophila)
Synonyms HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC014952
Gene Symbol TH1L
Gene ID (NCBI) 51497
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IXH7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C