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  • Featured Product
  • KD/KO Validated

THADA Polyclonal antibody

THADA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12909-1-AP

Print datasheet

Synonyms

tRNA (32-2'-O)-methyltransferase regulator THADA, Thyroid adenoma-associated protein, thyroid adenoma associated, KIAA1767, GITA

验证数据展示

  • WB analysis of CHO cells using 12909-1-AP

    WB analysis of CHO cells using 12909-1-AP

    WB result of 12909-1-AP from Takashi Kobayashi.

  • WB analysis of A549 using 12909-1-AP

    WB analysis of A549 using 12909-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 12909-1-AP

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 12909-1-AP

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 12909-1-AP

    IF Staining of A549 using 12909-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using THADA antibody (12909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, CHO cells
Positive IHC detected inhuman pancreas tissue, human testis tissue, mouse ovary tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12909-1-AP targets THADA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat, hamster
Cited Reactivityhuman, mouse
Immunogen THADA fusion protein Ag3881 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thyroid adenoma associated
Synonyms tRNA (32-2'-O)-methyltransferase regulator THADA, Thyroid adenoma-associated protein, thyroid adenoma associated, KIAA1767, GITA
Calculated Molecular Weight 220 kDa
Observed Molecular Weight 183 kDa
GenBank Accession NumberBC025773
Gene Symbol THADA
Gene ID (NCBI) 63892
RRIDAB_10638448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YHU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THADA antibody 12909-1-APDownload protocol
IHC protocol for THADA antibody 12909-1-APDownload protocol
IF protocol for THADA antibody 12909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li
  • KD Validated
View Article