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  • Featured Product
  • KD/KO Validated

THAP11 Polyclonal antibody

THAP11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23030-1-AP

Print datasheet

Synonyms

THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a

验证数据展示

  • IHC staining of human colon cancer using 23030-1-AP

    IHC staining of human colon cancer using 23030-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23030-1-AP (THAP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THAP11 antibody included
  • WB analysis using 23030-1-AP

    WB analysis using 23030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23030-1-AP (THAP11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, C2C12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy THAP11 Ready-To-Use IHC Kit. THAP11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23030-1-AP targets THAP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen THAP11 fusion protein Ag19342 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name THAP domain containing 11
Synonyms THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a
Calculated Molecular Weight 314 aa, 34 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC012182
Gene Symbol THAP11
Gene ID (NCBI) 57215
RRIDAB_2879200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96EK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THAP11 antibody 23030-1-APDownload protocol
IHC protocol for THAP11 antibody 23030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Res Int

THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways.

Authors - Jing Zhang
  • KD Validated
View Article
mouseWB,IF

iScience

Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei

Authors - Hui Rong Soon
View Article