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验证数据展示

  • IHC staining of human colon cancer using 23030-1-AP

    IHC staining of human colon cancer using 23030-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23030-1-AP (THAP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THAP11 antibody included
  • WB analysis using 23030-1-AP

    WB analysis using 23030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23030-1-AP (THAP11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

THAP11 Polyclonal antibody

THAP11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23030-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, C2C12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy THAP11 Ready-To-Use IHC Kit. THAP11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23030-1-AP targets THAP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 THAP11 fusion protein Ag19342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THAP domain containing 11
别名 THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a
计算分子量 314 aa, 34 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC012182
基因名称 THAP11
Gene ID (NCBI) 57215
RRIDAB_2879200
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96EK4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THAP11 antibody 23030-1-APDownload protocol
IHC protocol for THAP11 antibody 23030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Res Int

THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways.

Authors - Jing Zhang
  • KD Validated
View Article
mouseWB,IF

iScience

Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei

Authors - Hui Rong Soon
View Article