• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

THEM2 Polyclonal antibody

THEM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16782-1-AP

Print datasheet

Synonyms

EC:3.1.2.2, EC:3.1.2.-, Acyl-coenzyme A thioesterase 13, N-terminally processed, Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13

验证数据展示

  • WB analysis using 16782-1-AP

    WB analysis using 16782-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16782-1-AP

    IHC staining of human lung cancer using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IHC staining of human lung cancer using 16782-1-AP

    IHC staining of human lung cancer using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IF Staining of HepG2 using 16782-1-AP

    IF Staining of HepG2 using 16782-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using THEM2 antibody (16782-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IP experiment of HeLa using 16782-1-AP

    IP experiment of HeLa using 16782-1-AP

    IP result of anti-THEM2 (IP:16782-1-AP, 4ug; Detection:16782-1-AP 1:2000) with HeLa cells lysate 1085 ug.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACOT13/THEM2 Ready-To-Use IHC Kit. ACOT13/THEM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16782-1-AP targets THEM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen THEM2 fusion protein Ag10212 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioesterase superfamily member 2
Synonyms EC:3.1.2.2, EC:3.1.2.-, Acyl-coenzyme A thioesterase 13, N-terminally processed, Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13
Calculated Molecular Weight 140 aa, 15 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000894
Gene Symbol THEM2
Gene ID (NCBI) 55856
RRIDAB_2287241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Them2 (also named Acot13) is a homotetrameric fatty acyl-CoA thioesterase. Them2 is enriched in liver and oxidative tissues, including BAT, and is primarily associated with mitochondria.

实验方案

Product Specific Protocols
WB protocol for THEM2 antibody 16782-1-APDownload protocol
IHC protocol for THEM2 antibody 16782-1-APDownload protocol
IF protocol for THEM2 antibody 16782-1-APDownload protocol
IP protocol for THEM2 antibody 16782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols