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THG1L Polyclonal antibody

THG1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15208-1-AP

Print datasheet

Synonyms

ICF45, IHG-1, THG1L

验证数据展示

  • WB analysis of K-562 using 15208-1-AP

    WB analysis of K-562 using 15208-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15208-1-AP (THG1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 15208-1-AP

    IHC staining of human endometrial cancer using 15208-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15208-1-AP (THG1L antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15208-1-AP targets THG1L in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen THG1L fusion protein Ag7361 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
Synonyms ICF45, IHG-1, THG1L
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC001852
Gene Symbol THG1L
Gene ID (NCBI) 54974
RRIDAB_2303248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THG1L antibody 15208-1-APDownload protocol
IHC protocol for THG1L antibody 15208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Biochim Biophys Acta

Profibrotic IHG-1 complexes with renal disease associated HSPA5 and TRAP1 in mitochondria.

Authors - Una Bhreathnach
View Article