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验证数据展示

  • IP experiment of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

    IP experiment of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

    IP result of anti-THOC1 (IP:10920-1-AP, 4ug; Detection:10920-1-AP 1:500) with HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

    WB analysis of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10920-1-AP (same clone as 10920-1-PBS)

    IHC staining of human colon cancer using 10920-1-AP (same clone as 10920-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10920-1-AP (THOC1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with THOC1 antibody included
  • IF Staining of HeLa using 10920-1-AP (same clone as 10920-1-PBS)

    IF Staining of HeLa using 10920-1-AP (same clone as 10920-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC1 antibody (10920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

THOC1 Polyclonal antibody, PBS Only

THOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10920-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy THOC1 Ready-To-Use IHC Kit. THOC1 primary antibody included.

产品信息

10920-1-PBS targets THOC1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 THOC1 fusion protein Ag1352 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THO complex 1
别名
计算分子量 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC010381
基因名称 THOC1
Gene ID (NCBI) 9984
RRIDAB_2202239
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96FV9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy. This antibody recognizes the 84kd THOC1 protein.