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验证数据展示

  • WB analysis using 11716-1-AP

    WB analysis using 11716-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11716-1-AP (THOC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 11716-1-AP

    IHC staining of human ovary cancer using 11716-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11716-1-AP (THOC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

THOC3 Polyclonal antibody

THOC3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11716-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Tho3, TEX1, hTREX45

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11716-1-AP targets THOC3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 THOC3 fusion protein Ag2289 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THO complex 3
别名 Tho3, TEX1, hTREX45
计算分子量 327 aa, 36 kDa
观测分子量39 kDa
GenBank蛋白编号BC006849
基因名称 THOC3
Gene ID (NCBI) 84321
RRIDAB_3085379
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96J01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THOC3 is a component of the nuclear THO transcription elongation complex, which is a part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. Additionally, THOC3 plays a key role in many cellular mechanisms, such as in the pathogenesis of cancer and neurodegenerative diseases (PMID: 36674977). THOC3 has 2 isoforms with the molecular weights of 36 and 39 kDa.

实验方案

Product Specific Protocols
WB protocol for THOC3 antibody 11716-1-APDownload protocol
IHC protocol for THOC3 antibody 11716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols