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验证数据展示

  • WB analysis using 14862-1-AP

    WB analysis using 14862-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 14862-1-AP

    IP experiment of HEK-293 using 14862-1-AP

    IP result of anti-THOC5 (IP:14862-1-AP, 4ug; Detection:14862-1-AP 1:5000) with HEK-293 cells lysate 920 ug.

  • IHC staining of human breast cancer using 14862-1-AP

    IHC staining of human breast cancer using 14862-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THOC5 antibody included
  • IHC staining of human breast cancer using 14862-1-AP

    IHC staining of human breast cancer using 14862-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THOC5 antibody included
  • IHC staining of human lung cancer using 14862-1-AP

    IHC staining of human lung cancer using 14862-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THOC5 antibody included
  • IF Staining of HepG2 using 14862-1-AP

    IF Staining of HepG2 using 14862-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using THOC5 antibody (14862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

THOC5 Polyclonal antibody

THOC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14862-1-AP

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Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIAA0983, Functional spliceosome-associated protein 79, C22orf19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy THOC5 Ready-To-Use IHC Kit. THOC5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 1 publications below

产品信息

14862-1-AP targets THOC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen THOC5 fusion protein Ag6664 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name THO complex 5
Synonyms KIAA0983, Functional spliceosome-associated protein 79, C22orf19
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC003615
Gene Symbol THOC5
Gene ID (NCBI) 8563
RRIDAB_2878088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THOC5, also named as C22orf19, or KIAA0983, is a 683 amino acid protein, which belongs to the THOC5 family. THOC5 is ubiquitously expressed in many tissues. THOC5 localizes in the nucleus and acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA.THOC5 plays a role in cytokine-stimulated monocytic development and affects granulocyte/macrophage differentiation.

实验方案

Product Specific Protocols
WB protocol for THOC5 antibody 14862-1-APDownload protocol
IHC protocol for THOC5 antibody 14862-1-APDownload protocol
IF protocol for THOC5 antibody 14862-1-APDownload protocol
IP protocol for THOC5 antibody 14862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Cell Stem Cell

The THO Complex Regulates Pluripotency Gene mRNA Export and Controls Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming.

Authors - Wang Li L
  • KD Validated
View Article