• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

THOC6 Polyclonal antibody

THOC6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15316-1-AP

Print datasheet

Synonyms

fSAP35, THO complex subunit 6 homolog, THOC6, WDR58

验证数据展示

  • WB analysis using 15316-1-AP

    WB analysis using 15316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 15316-1-AP

    IP experiment of A431 using 15316-1-AP

    IP result of anti-THOC6 (IP:15316-1-AP, 4ug; Detection:15316-1-AP 1:300) with A431 cells lysate 2280 ug.

  • IHC staining of human ovary tumor using 15316-1-AP

    IHC staining of human ovary tumor using 15316-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15316-1-AP (THOC6 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA431 cells, mouse ovary tissue, Neuro-2a cells
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15316-1-AP targets THOC6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen THOC6 fusion protein Ag7561 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name THO complex 6 homolog (Drosophila)
Synonyms fSAP35, THO complex subunit 6 homolog, THOC6, WDR58
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC003118
Gene Symbol THOC6
Gene ID (NCBI) 79228
RRIDAB_2240482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86W42
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THOC6 antibody 15316-1-APDownload protocol
IHC protocol for THOC6 antibody 15316-1-APDownload protocol
IP protocol for THOC6 antibody 15316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Viruses

Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.

Authors - Toshie Sakuma
  • KD Validated
View Article