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THOP1 Polyclonal antibody

THOP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11738-1-AP

Print datasheet

Synonyms

Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP

验证数据展示

  • WB analysis using 11738-1-AP

    WB analysis using 11738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11738-1-AP (THOP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11738-1-AP

    IHC staining of human colon cancer using 11738-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11738-1-AP (THOP1 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis using 11738-1-AP

    WB analysis using 11738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11738-1-AP (THOP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 11738-1-AP

    IP experiment of human placenta using 11738-1-AP

    IP result of anti-THOP1 (IP:11738-1-AP, 4ug; Detection:11738-1-AP 1:500) with human placenta tissue lysate 1520ug.

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11738-1-AP targets THOP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen THOP1 fusion protein Ag2268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thimet oligopeptidase 1
Synonyms Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC000135
Gene Symbol THOP1
Gene ID (NCBI) 7064
RRIDAB_2303269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides

实验方案

Product Specific Protocols
WB protocol for THOP1 antibody 11738-1-APDownload protocol
IHC protocol for THOP1 antibody 11738-1-APDownload protocol
IP protocol for THOP1 antibody 11738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols