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THOP1 Monoclonal antibody

THOP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse , Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B11A11

Cat no : 68587-1-Ig

Print datasheet

Synonyms

Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP

验证数据展示

  • WB analysis using 68587-1-Ig

    WB analysis using 68587-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68587-1-Ig (THOP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HeLa using 68587-1-Ig

    WB analysis of HeLa using 68587-1-Ig

    WB result of THOP1 antibody (68587-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THOP1 transfected HeLa cells.

  • WB analysis using 68587-1-Ig

    WB analysis using 68587-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68587-1-Ig (THOP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, rat brain tissue, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68587-1-Ig targets THOP1 in WB, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse , Rat
Immunogen THOP1 fusion protein Ag2268 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name thimet oligopeptidase 1
Synonyms Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC000135
Gene Symbol THOP1
Gene ID (NCBI) 7064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides

实验方案

Product Specific Protocols
WB protocol for THOP1 antibody 68587-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols