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THOP1 Recombinant antibody, PBS Only (Detector)

THOP1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241607G8

Cat no : 84319-3-PBS

Print datasheet

Synonyms

THOP 1, MP78, MEPD_HUMAN, EP24.15, EC:3.4.24.15

验证数据展示

  • WB analysis using 84319-3-RR (same clone as 84319-3-PBS)

    WB analysis using 84319-3-RR (same clone as 84319-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84319-3-RR (THOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84319-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01191-3

    Sandwich ELISA standard curve of MP01191-3

    Sandwich ELISA standard curve of MP01191-3, Human THOP1 Recombinant Matched Antibody Pair - PBS only. 84319-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2268. 84319-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Sample test of MP01191-3

    Sample test of MP01191-3

    The mean THOP1 concentration was determined to be 154.80 ng/mL in THP-1 cell extract based on a 4.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP01191-1

    Cytometric bead array standard curve of MP01191-1

    Cytometric bead array standard curve of MP01191-1, THOP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84319-4-PBS. Detection antibody: 84319-3-PBS. Standard: Ag2268. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01191-3

    Cytometric bead array standard curve of MP01191-3

    Cytometric bead array standard curve of MP01191-3, THOP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84319-2-PBS. Detection antibody: 84319-3-PBS. Standard: Ag2268. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84319-3-RR

    Affinity and Kinetic Characterization of 84319-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84319-3-RR against Human THOP1 were performed. The affinity constant is below 1 pM.

产品信息

84319-3-PBS targets THOP1 as part of a matched antibody pair:

MP01191-1: 84319-4-PBS capture and 84319-3-PBS detection (validated in Cytometric bead array)

MP01191-3: 84319-2-PBS capture and 84319-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen THOP1 fusion protein Ag2268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name thimet oligopeptidase 1
Synonyms THOP 1, MP78, MEPD_HUMAN, EP24.15, EC:3.4.24.15
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC000135
Gene Symbol THOP1
Gene ID (NCBI) 7064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52888
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides