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THPO Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG2b

Detection: Mouse / IgG1

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP50053-1

Print datasheet

Synonyms

C mpl ligand, MGDF, MKCSF, ML, MPLLG, THPO, thrombopoietin, TPO

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50053-1, THPO Monoclonal Matched Antibody Pair, PBS Only. 68672-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1248. 68672-2-PBS was HRP conjugated as detection antibody. Range: 15.6-1000 pg/mL.

    Sandwich ELISA standard curve of MP50053-1

    Sandwich ELISA standard curve of MP50053-1, THPO Monoclonal Matched Antibody Pair, PBS Only. 68672-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1248. 68672-2-PBS was HRP conjugated as detection antibody. Range: 15.6-1000 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP50053-1 targets THPO in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: Thrombopoietin Monoclonal antibody, PBS Only (Capture) 68672-1-PBS (1B7D1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Thrombopoietin Monoclonal antibody, PBS Only (Detector) 68672-2-PBS (1A8G2). 100 μg. Concentration 1 mgl/ml.

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 68672-1-PBS
Tested Reactivity Human
Immunogen Thrombopoietin fusion protein HZ-1248
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=2.84 x 10-9M
KOff=3.32 x 10-4M
KOn=1.17 x 105M
Full Name thrombopoietin
Synonyms C mpl ligand, MGDF, MKCSF, ML, MPLLG, THPO, thrombopoietin, TPO
Gene symbol THPO
Gene ID (NCBI) 7066
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 68672-2-PBS
Tested Reactivity Human
Immunogen Thrombopoietin fusion protein HZ-1248
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.85 x 10-9M
KOff=3.08 x 10-4M
KOn=1.67 x 105M
Full Name thrombopoietin
Synonyms C mpl ligand, MGDF, MKCSF, ML, MPLLG, THPO, thrombopoietin, TPO
Gene symbol THPO
UniprotP40225
Gene ID (NCBI) 7066
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.