验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:100-1:600 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
13054-1-AP targets THRSP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | THRSP fusion protein Ag3721 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thyroid hormone responsive (SPOT14 homolog, rat) |
| 别名 | S14, Spot 14 protein, SPOT14, THRSP |
| 计算分子量 | 146 aa, 17 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC031989 |
| 基因名称 | THRSP |
| Gene ID (NCBI) | 7069 |
| RRID | AB_2877906 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92748 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
THRSP (Thyroid hormone-inducible hepatic protein) is a protein that in humans is encoded by the THRSP gene. The protein encoded by this gene is similar to the gene product of S14, a rat gene whose expression is limited to liver and adipose tissue and is controlled by nutritional and hormonal factors. This gene has been shown to be expressed in liver and adipocytes, particularly in lipomatous modules. It is also found to be expressed in lipogenic breast cancers, which suggests a role in controlling tumor lipid metabolism (RefSeq). This antibody works well in IHC, WB and IF.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for THRSP antibody 13054-1-AP | Download protocol |
| IHC protocol for THRSP antibody 13054-1-AP | Download protocol |
| IP protocol for THRSP antibody 13054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Med Insulin-inducible THRSP maintains mitochondrial function and regulates sphingolipid metabolism in human adipocytes. | ||
Sci Rep Elevated serum S14 levels are associated with more severe liver steatosis by ultrasonography. | ||
Mol Cell Endocrinol MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.
| ||
Medicine (Baltimore) Serum Spot 14 concentration is negatively associated with thyroid-stimulating hormone level. | ||
PLoS One The serum level of a novel lipogenic protein Spot 14 was reduced in metabolic syndrome. | ||
Adv Sci (Weinh) Spatial Transcriptomic Study Reveals Heterogeneous Metabolic Adaptation and a Role of Pericentral PPARα/CAR/Ces2a Axis During Fasting in Mouse Liver |
