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THTPA Polyclonal antibody

THTPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15486-1-AP

Print datasheet

Synonyms

ThTPase, THTP, Thiamine-triphosphatase, thiamine triphosphatase, EC:3.6.1.28

验证数据展示

  • WB analysis of K-562 using 15486-1-AP

    WB analysis of K-562 using 15486-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15486-1-AP

    IHC staining of human stomach cancer using 15486-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 15486-1-AP

    IF Staining of HepG2 using 15486-1-AP

    Immunofluorescent analysis of HepG2 cells, using THTPA antibody 15486-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of K-562 using 15486-1-AP

    IP experiment of K-562 using 15486-1-AP

    IP result of anti-THTPA (IP:15486-1-AP, 3ug; Detection:15486-1-AP 1:800) with K-562 cells lysate 1720ug.

经过测试的应用

Positive WB detected inK-562 cells, A431 cells, HeLa cells, human brain tissue, mouse testis tissue, mouse uterus tissue, PC-3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15486-1-AP targets THTPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen THTPA fusion protein Ag7834 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thiamine triphosphatase
Synonyms ThTPase, THTP, Thiamine-triphosphatase, thiamine triphosphatase, EC:3.6.1.28
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26-35 kDa
GenBank Accession NumberBC002984
Gene Symbol THTPA
Gene ID (NCBI) 79178
RRIDAB_2203183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU02
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THTPA antibody 15486-1-APDownload protocol
IHC protocol for THTPA antibody 15486-1-APDownload protocol
IF protocol for THTPA antibody 15486-1-APDownload protocol
IP protocol for THTPA antibody 15486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

Authors - Shuqian Liu
View Article