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验证数据展示

  • WB analysis using 21054-1-AP

    WB analysis using 21054-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21054-1-AP (THUMPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21054-1-AP

    IF Staining of HeLa using 21054-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THUMPD2 antibody (21054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

THUMPD2 Polyclonal antibody

THUMPD2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21054-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

THUMP domain-containing protein 2, THUMP domain containing 2, C2orf8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21054-1-AP targets THUMPD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 THUMPD2 fusion protein Ag13736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THUMP domain containing 2
别名 THUMP domain-containing protein 2, THUMP domain containing 2, C2orf8
计算分子量 473 aa, 53 kDa
观测分子量56 kDa
GenBank蛋白编号BC004163
基因名称 THUMPD2
Gene ID (NCBI) 80745
RRIDAB_3085634
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THUMP domain-containing protein 2 (THUMPD2) is the methyltransferase, which interacts with an auxiliary protein, TRMT112, to catalyze U6 m2G72 formation. Western blot analysis detected THUMPD2 at an apparent molecular mass of 56 kDa.

实验方案

Product Specific Protocols
WB protocol for THUMPD2 antibody 21054-1-APDownload protocol
IF protocol for THUMPD2 antibody 21054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols