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验证数据展示

  • WB analysis using 15867-1-AP

    WB analysis using 15867-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 15867-1-AP

    IHC staining of human lung cancer using 15867-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 15867-1-AP

    IHC staining of human lung cancer using 15867-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 15867-1-AP

    IF Staining of HepG2 using 15867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15867-1-AP (THYN1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

THYN1 Polyclonal antibody

THYN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15867-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

THY28KD, THY28, MY105, My0054, MDS012

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15867-1-AP targets THYN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 THYN1 fusion protein Ag8481 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thymocyte nuclear protein 1
别名 THY28KD, THY28, MY105, My0054, MDS012
计算分子量225aa,26 kDa; 166aa,19 kDa
观测分子量 28-32 kDa
GenBank蛋白编号BC006978
基因名称 THYN1
Gene ID (NCBI) 29087
RRIDAB_2287179
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P016
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THYN1, also named as THY28, is a 28-32 kDa protein which located mainly in the nucleus. It appears to correlate with induction of apoptosis. (PMID:14601557)

实验方案

Product Specific Protocols
WB protocol for THYN1 antibody 15867-1-APDownload protocol
IHC protocol for THYN1 antibody 15867-1-APDownload protocol
IF protocol for THYN1 antibody 15867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols