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TIA1 Polyclonal antibody

TIA1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 12133-2-AP

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Synonyms

RNA-binding protein TIA-1, RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40



经过测试的应用

Positive WB detected inCOLO 320 cells, Raji cells, mouse spleen tissue, mouse thymus tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells, HepG2 cells, HeLa cells, sodium arsenite treated HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TIA1 Ready-To-Use IHC Kit. TIA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12133-2-AP targets TIA1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP, ChIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken
Immunogen TIA1 fusion protein Ag2778 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TIA1 cytotoxic granule-associated RNA binding protein
Synonyms RNA-binding protein TIA-1, RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40
Calculated Molecular Weight 214 aa, 24 kDa, 43 kDa
Observed Molecular Weight ~40 kDa
GenBank Accession NumberBC015944
Gene Symbol TIA1
Gene ID (NCBI) 7072
RRIDAB_2201427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31483
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43. Moreover, TDP-43 in SGs became less mobile and insoluble.

实验方案

Product Specific Protocols
WB protocol for TIA1 antibody 12133-2-APDownload protocol
IHC protocol for TIA1 antibody 12133-2-APDownload protocol
IF protocol for TIA1 antibody 12133-2-APDownload protocol
IP protocol for TIA1 antibody 12133-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
  • KD Validated
humanRIP

Cell Res

Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures.

Authors - Lei Sun
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanIF,IHC

Neuron

TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics.

Authors - Ian R Mackenzie
mouseWB

Nat Commun

Cellular stress alters 3'UTR landscape through alternative polyadenylation and isoform-specific degradation.

Authors - Dinghai Zheng
mouseWB

Brain

Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia

Authors - Laura Pasetto