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验证数据展示

  • WB analysis using 17649-1-AP

    WB analysis using 17649-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17649-1-AP (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 17649-1-AP

    IHC staining of human stomach using 17649-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17649-1-AP (TIAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TIAL1 antibody included
  • Featured Product
  • KD/KO验证

TIAR Polyclonal antibody

TIAR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17649-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TIAL1 Ready-To-Use IHC Kit. TIAL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17649-1-AP targets TIAR in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 TIAR fusion protein Ag11884 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TIA1 cytotoxic granule-associated RNA binding protein-like 1
别名 TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR
计算分子量 42 kDa
观测分子量38-43 kDa
GenBank蛋白编号BC030025
基因名称 TIAR
Gene ID (NCBI) 7073
RRIDAB_3669280
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites.. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)

实验方案

Product Specific Protocols
WB protocol for TIAR antibody 17649-1-APDownload protocol
IHC protocol for TIAR antibody 17649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.

Authors - Wenhui Zhao
  • KD Validated
View Article