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  • Featured Product
  • KD/KO Validated

TIAR Polyclonal antibody

TIAR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17649-1-AP

Print datasheet

Synonyms

TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR

验证数据展示

  • WB analysis using 17649-1-AP

    WB analysis using 17649-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17649-1-AP (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 17649-1-AP

    IHC staining of human stomach using 17649-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17649-1-AP (TIAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TIAL1 antibody included

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TIAL1 Ready-To-Use IHC Kit. TIAL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17649-1-AP targets TIAR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TIAR fusion protein Ag11884 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TIA1 cytotoxic granule-associated RNA binding protein-like 1
Synonyms TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR
Calculated Molecular Weight 42 kDa
Observed Molecular Weight38-43 kDa
GenBank Accession NumberBC030025
Gene Symbol TIAR
Gene ID (NCBI) 7073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites.. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)

实验方案

Product Specific Protocols
WB protocol for TIAR antibody 17649-1-APDownload protocol
IHC protocol for TIAR antibody 17649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.

Authors - Wenhui Zhao
  • KD Validated
View Article