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TIAM2 Polyclonal antibody

TIAM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26870-1-AP

Print datasheet

Synonyms

T-lymphoma invasion and metastasis-inducing protein 2, TIAM-2, TIAM 2, STEF, SIF and TIAM1-like exchange factor

验证数据展示

  • WB analysis using 26870-1-AP

    WB analysis using 26870-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 26870-1-AP

    IF Staining of RAW 264.7 using 26870-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of K-562 using 26870-1-AP

    FC experiment of K-562 using 26870-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inBxPC-3 cells, U2OS cells, mouse testis tissue, rat testis tissue, HEK-293 cells
Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26870-1-AP targets TIAM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TIAM2 fusion protein Ag25469 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name T-cell lymphoma invasion and metastasis 2
Synonyms T-lymphoma invasion and metastasis-inducing protein 2, TIAM-2, TIAM 2, STEF, SIF and TIAM1-like exchange factor
Observed Molecular Weight190-200 kDa
GenBank Accession NumberBC137275
Gene Symbol TIAM2
Gene ID (NCBI) 26230
RRIDAB_3085914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVF5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.

实验方案

Product Specific Protocols
WB protocol for TIAM2 antibody 26870-1-APDownload protocol
IF protocol for TIAM2 antibody 26870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols