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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

    WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 66907-1-Ig (same clone as 66907-1-PBS)

    WB analysis of RAW 264.7 using 66907-1-Ig (same clone as 66907-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

    IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TIAL1 antibody included
  • IF Staining of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

    IF Staining of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66907-1-Ig (TIAR antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

    WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

    FC experiment of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human TIAR (66907-1-Ig, Clone:1E8D12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

TIAR Monoclonal antibody, PBS Only

TIAR Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66907-1-PBS

产品说明书

CloneNo. 1E8D12

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TIAL1 Ready-To-Use IHC Kit. TIAL1 primary antibody included.

产品信息

66907-1-PBS targets TIAR in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 TIAR fusion protein Ag11981 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TIA1 cytotoxic granule-associated RNA binding protein-like 1
别名 TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR
计算分子量 42 kDa
观测分子量 39 and 41 kDa
GenBank蛋白编号BC030025
基因名称 TIAR
Gene ID (NCBI) 7073
RRIDAB_2882234
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ01085
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites. 66907-1-Ig detects 39 and 41 kDa bands in SDS-PAGE. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)