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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 98023-2-RR (same clone as 98023-2-PBS)

    FC experiment of human PBMCs using 98023-2-RR (same clone as 98023-2-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98023-2-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98023-2-RR (same clone as 98023-2-PBS)

    FC experiment of human PBMCs using 98023-2-RR (same clone as 98023-2-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98023-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98023-2-RR

    Affinity and Kinetic Characterization of 98023-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98023-2-RR against Human TIGIT were performed. The affinity constant is 5.45 nM.

Anti-Human TIGIT Rabbit Recombinant Antibody, PBS Only

TIGIT Uni-rAbTM Recombinant Antibody for FC
Cat No. 98023-2-PBS

产品说明书

CloneNo. 240423C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

240423C7, T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98023-2-PBS targets TIGIT in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 T cell immunoreceptor with Ig and ITIM domains
别名 240423C7, T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3
计算分子量26 kDa
GenBank蛋白编号NM_173799.4
基因名称 TIGIT
Gene ID (NCBI) 201633
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ495A1-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).