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Anti-Human TIGIT Rabbit Recombinant Antibody

TIGIT Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240423A7

Cat no : 98023-3-RR

Print datasheet

Synonyms

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

验证数据展示

  • FC experiment of human PBMCs using 98023-3-RR

    FC experiment of human PBMCs using 98023-3-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-3-RR, Clone: 240423A7) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98023-3-RR

    Affinity and Kinetic Characterization of 98023-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98023-3-RR against Human TIGIT were performed. The affinity constant is 4.00 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98023-3-RR is TIGIT Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name T cell immunoreceptor with Ig and ITIM domains
Synonyms VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
Calculated Molecular Weight26 kDa
GenBank Accession NumberNM_173799.4
Gene Symbol TIGIT
Gene ID (NCBI) 201633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ495A1-1
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

实验方案

Product Specific Protocols
FC protocol for TIGIT antibody 98023-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols