APC Anti-Mouse TIGIT (1G9)
TIGIT Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
mouse
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
1G9
验证数据展示
经过测试的应用
| Positive FC detected in | plate-bound Anti-CD3 and Anti-CD28 stimulated BALB/c mouse splenocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-65079 targets TIGIT in FC applications and shows reactivity with mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | mouse |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | T cell immunoreceptor with Ig and ITIM domains |
| Synonyms | Vstm3, 1G9 |
| GenBank Accession Number | NM_001146325 |
| Gene Symbol | Tigit |
| Gene ID (NCBI) | 100043314 |
| RRID | AB_2882978 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P86176 |
| Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC TIGIT antibody APC-65079 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
