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验证数据展示

  • IF Staining of human tonsillitis using 60355-1-Ig (same clone as 60355-1-PBS)

    IF Staining of human tonsillitis using 60355-1-Ig (same clone as 60355-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TIM3 antibody (60355-1-Ig, Clone: 4C4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • WB analysis of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

    WB analysis of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

TIM3 Monoclonal antibody, PBS Only

TIM3 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 60355-1-PBS

产品说明书

CloneNo. 4C4G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, ELISA

别名

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60355-1-PBS targets TIM3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TIM3 fusion protein Ag16901 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatitis A virus cellular receptor 2
别名 T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366
计算分子量 301 aa, 33 kDa
观测分子量 33 kDa, 50-70 kDa
GenBank蛋白编号BC020843
基因名称 TIM3
Gene ID (NCBI) 84868
RRIDAB_2881464
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDQ0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).