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TIM3 Monoclonal antibody, PBS Only

TIM3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C4G3

Cat no : 60355-1-PBS

Print datasheet

Synonyms

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3

验证数据展示

  • WB analysis of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

    WB analysis of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

产品信息

60355-1-PBS targets TIM3 in WB, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, pig, mouse, rat
Immunogen TIM3 fusion protein Ag16901 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hepatitis A virus cellular receptor 2
Synonyms CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 33 kDa, 50-70 kDa
GenBank Accession NumberBC020843
Gene Symbol TIM3
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDQ0
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).