验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, MCF-7 cells, Neuro-2a cells, NIH/3T3 cells, U-251 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 2 publications below |
产品信息
11189-1-AP targets TIMM17A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, rat |
免疫原 | TIMM17A fusion protein Ag1665 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | translocase of inner mitochondrial membrane 17 homolog A (yeast) |
别名 | MIMT17, TIM17, TIM17A, TIMM17, TIMM17A |
计算分子量 | 18 kDa |
观测分子量 | 18 kDa |
GenBank蛋白编号 | BC015098 |
基因名称 | TIMM17A |
Gene ID (NCBI) | 10440 |
RRID | AB_2271661 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99595 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for TIMM17A antibody 11189-1-AP | Download protocol |
IHC protocol for TIMM17A antibody 11189-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria | ||
EMBO Rep UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation | ||
J Mol Cell Cardiol Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress. | ||
Proteomics Quantitative proteomics study of breast cancer cell lines isolated from a single patient: discovery of TIMM17A as a marker for breast cancer.
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Int J Mol Med 'Governor vessel-unblocking and mind-regulating' acupuncture therapy ameliorates cognitive dysfunction in a rat model of middle cerebral artery occlusion. | ||
J Cell Biol ROMO1 is a constituent of the human presequence translocase required for YME1L protease import. |