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TIM22 Polyclonal antibody

TIM22 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14927-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

Testis expressed sequence 4, TEX4, TIM22, TIMM22

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14927-1-AP targets TIM22 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TIM22 fusion protein Ag6689 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 22 homolog (yeast)
别名 Testis expressed sequence 4, TEX4, TIM22, TIMM22
计算分子量 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC002324
基因名称 TIM22
Gene ID (NCBI) 29928
RRIDAB_11183050
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y584
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIM22 is a translocase localized in mitochondrial inner membrane. It is implicated in import of nuclear-encoded mitochondrial preproteins to the mitochondria. TIM22 is the essential core of protein insertion complex (TIM22 complex) that consists of TIM22, TIM54, TIM18, and TIM12.

实验方案

Product Specific Protocols
WB protocol for TIM22 antibody 14927-1-APDownload protocol
IHC protocol for TIM22 antibody 14927-1-APDownload protocol
IP protocol for TIM22 antibody 14927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.

Authors - Atsushi Hoshino
humanWB

Nat Med

The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.

Authors - Wenzhang Wang
  • KD Validated
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
mouseWB

Cell Metab

Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.

Authors - Zhilin Hu
humanWB

Cell Metab

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

Authors - Marcel Morgenstern
humanWB

Mol Cell

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.

Authors - Milena Vukotic
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