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TIMP1 Polyclonal antibody

TIMP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30755-1-AP

Print datasheet

Synonyms

TIMP-1, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA

验证数据展示

  • WB analysis using 30755-1-AP

    WB analysis using 30755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30755-1-AP (TIMP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 30755-1-AP

    IHC staining of rat testis using 30755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30755-1-AP (TIMP1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of rat testis using 30755-1-AP

    IHC staining of rat testis using 30755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30755-1-AP (TIMP1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 30755-1-AP

    IF Staining of HepG2 using 30755-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TIMP1 antibody (30755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 30755-1-AP

    IF Staining of HeLa using 30755-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (30755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HT-29 using 30755-1-AP

    FC experiment of HT-29 using 30755-1-AP

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human TIMP1 (30755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA2780 cells, HT-29 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30755-1-AP targets TIMP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TIMP metallopeptidase inhibitor 1
Synonyms TIMP-1, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-28 kDa
GenBank Accession NumberBC000866
Gene Symbol TIMP1
Gene ID (NCBI) 7076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

实验方案

Product Specific Protocols
WB protocol for TIMP1 antibody 30755-1-APDownload protocol
IHC protocol for TIMP1 antibody 30755-1-APDownload protocol
IF protocol for TIMP1 antibody 30755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols