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TIMP1 Recombinant antibody

TIMP1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230329E7

Cat no : 83151-5-RR

Print datasheet

Synonyms

TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Collagenase inhibitor

验证数据展示

  • IF Staining of HeLa using 83151-5-RR

    IF Staining of HeLa using 83151-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of HeLa using 83151-5-RR

    IF Staining of HeLa using 83151-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HT-29 using 83151-5-RR

    FC experiment of HT-29 using 83151-5-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of MDA-MB-231 using 83151-5-RR

    FC experiment of MDA-MB-231 using 83151-5-RR

    1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83151-5-RR

    Affinity and Kinetic Characterization of 83151-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83151-5-RR against Human TIMP1 were performed. The affinity constant is 1.33 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHT-29 cells, MDA-MB-231 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83151-5-RR is TIMP1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TIMP metallopeptidase inhibitor 1
Synonyms TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Collagenase inhibitor
Calculated Molecular Weight 23 kDa
GenBank Accession NumberBC000866
Gene Symbol TIMP1
Gene ID (NCBI) 7076
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

实验方案

Product Specific Protocols
IF protocol for TIMP1 antibody 83151-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols