验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| ChIP | See 2 publications below |
产品信息
11368-1-AP targets TIN2 in WB, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, chip |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | TIN2 fusion protein Ag1922 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TERF1 (TRF1)-interacting nuclear factor 2 |
| 别名 | TINF2, TRF1-interacting nuclear protein 2, TIN2L, TERF1-interacting nuclear factor 2 |
| 计算分子量 | 451 aa, 50 kDa |
| 观测分子量 | 50 kDa, 40 kDa |
| GenBank蛋白编号 | BC005030 |
| 基因名称 | TINF2 |
| Gene ID (NCBI) | 26277 |
| RRID | AB_2205100 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BSI4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TIN2, also named as TINF2, is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TIN2 has some isoforms with MW 50 kDa (TIN2L), 40 kDa (TIN2S) and 13 kDa. This antibody detects TIN2L, TIN2S and the shelterin complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TIN2 antibody 11368-1-AP | Download protocol |
| IF protocol for TIN2 antibody 11368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget BRCA1/2 mutations perturb telomere biology: characterization of structural and functional abnormalities in vitro and in vivo. | ||
Tissue Cell IGF2BPs-regulated TIN2 confers the malignant biological behaviors of gastric cancer cells | ||
Nat Commun Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence |