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TIN2 Polyclonal antibody

TIN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11368-1-AP

Print datasheet

Synonyms

TINF2, TRF1-interacting nuclear protein 2, TIN2L, TERF1-interacting nuclear factor 2

验证数据展示

  • WB analysis of mouse spleen using 11368-1-AP

    WB analysis of mouse spleen using 11368-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11368-1-AP (TIN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11368-1-AP

    IF Staining of HepG2 using 11368-1-AP

    Immunofluorescent analysis of HepG2 cells, using TINF2 antibody 11368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11368-1-AP targets TIN2 in WB, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, chip
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TIN2 fusion protein Ag1922 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TERF1 (TRF1)-interacting nuclear factor 2
Synonyms TINF2, TRF1-interacting nuclear protein 2, TIN2L, TERF1-interacting nuclear factor 2
Calculated Molecular Weight 451 aa, 50 kDa
Observed Molecular Weight 50 kDa, 40 kDa
GenBank Accession NumberBC005030
Gene Symbol TINF2
Gene ID (NCBI) 26277
RRIDAB_2205100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSI4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIN2, also named as TINF2, is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TIN2 has some isoforms with MW 50 kDa (TIN2L), 40 kDa (TIN2S) and 13 kDa. This antibody detects TIN2L, TIN2S and the shelterin complex.

实验方案

Product Specific Protocols
WB protocol for TIN2 antibody 11368-1-APDownload protocol
IF protocol for TIN2 antibody 11368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchip

Oncotarget

BRCA1/2 mutations perturb telomere biology: characterization of structural and functional abnormalities in vitro and in vivo.

Authors - Orit Uziel
View Article
human

Cells

The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells.

Authors - Tomer Harpaz
View Article
humanWB

Sci Adv

Structural basis of telomeric nucleosome recognition by shelterin factor TRF1

Authors - Hongmiao Hu
View Article
humanWB,ChIP

Nat Commun

Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence

Authors - Athanasios Siametis
View Article