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验证数据展示

  • WB analysis of mouse small intestine using 19747-1-AP

    WB analysis of mouse small intestine using 19747-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 19747-1-AP

    IHC staining of human colon using 19747-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 19747-1-AP

    IHC staining of human colon using 19747-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 40x lens).

TINAG-Specific Polyclonal antibody

TINAG-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19747-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

TIN AG, TINAG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse small intestine tissue, human kidney tissue, human placenta tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

19747-1-AP targets TINAG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulointerstitial nephritis antigen
别名 TIN AG, TINAG
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号NM_014464
基因名称 TINAG
Gene ID (NCBI) 27283
RRIDAB_10642433
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJW2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TINAG belongs to the peptidase C1 family. TINAG mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. It is a non catalytic peptidase C1 family protein. Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.It has 2 isoforms produced by alternative splicing. The antibody is specific to TINAG.

实验方案

Product Specific Protocols
WB protocol for TINAG-Specific antibody 19747-1-APDownload protocol
IHC protocol for TINAG-Specific antibody 19747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Transl Cancer Res

Modeling of new markers for the diagnosis and prognosis of pancreatic cancer based on the transition from inflammation to cancer

Authors - Yuan Zhou
View Article