验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, human adrenal gland tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1500 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12077-1-AP targets TINAGL1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TINAGL1 fusion protein Ag2721 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tubulointerstitial nephritis antigen-like 1 |
| 别名 | PP6614, Oxidized LDL-responsive gene 2 protein, OLRG-2, LCN7, Glucocorticoid-inducible protein 5 |
| 计算分子量 | 467 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC009048 |
| 基因名称 | TINAGL1 |
| Gene ID (NCBI) | 64129 |
| RRID | AB_2058942 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZM7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| IHC protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| IF protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| IP protocol for TINAGL1 antibody 12077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Cell Res Functional analysis reveals that Tinagl1 is required for normal muscle development in mice through the activation of ERK signaling.
| ||
J Transl Med Tubulointerstitial nephritis antigen-like 1 from cancer-associated fibroblasts contribute to the progression of diffuse-type gastric cancers through the interaction with integrin β1 | ||
Cell Signal YTHDF1 facilitates esophageal cancer progression via augmenting m6A-dependent TINAGL1 translation
| ||
Matrix Biol Plus Matrisome proteomics reveals novel mediators of muscle remodeling with aerobic exercise training | ||
Int J Biol Sci Tubulointerstitial nephritis antigen-like 1 promotes the progression of liver fibrosis after HCV eradication with direct-acting antivirals |
