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验证数据展示

  • IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • WB analysis using 66523-1-Ig (same clone as 66523-1-PBS)

    WB analysis using 66523-1-Ig (same clone as 66523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 66523-1-Ig (same clone as 66523-1-PBS)

    WB analysis of U2OS using 66523-1-Ig (same clone as 66523-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

Perilipin 3/TIP47 Monoclonal antibody, PBS Only

Perilipin 3/TIP47 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66523-1-PBS

产品说明书

CloneNo. 4C11B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

M6PRBP1, Perilipin 3, TIP47, Mannose-6-phosphate receptor-binding protein 1, 4C11B1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66523-1-PBS targets Perilipin 3/TIP47 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Perilipin 3/TIP47 fusion protein Ag1028 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mannose-6-phosphate receptor binding protein 1
别名 M6PRBP1, Perilipin 3, TIP47, Mannose-6-phosphate receptor-binding protein 1, 4C11B1
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC007566
基因名称 TIP47/PLIN3
Gene ID (NCBI) 10226
RRIDAB_2881886
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60664
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.